The Research of Ovarian Proteomics of Dolang Sheep at Different Physiological Periods Based on iTRAQ Technology | Cui | Animal Molecular Breeding

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The Research of Ovarian Proteomics of Dolang Sheep at Different Physiological Periods Based on iTRAQ Technology  

Zilong Cui , Shuangyang Wang , Xueliang Yang , Tuersuntuoheti Mirenisa , Weihua Chang
1 College of Animal Science, Tarim University, Alar, Xinjiang 843300
2 Key Laboratory of the Corps of Tarim Animal Husbandry Science and Technology, Alar, Xinjiang 843300
Author    Correspondence author
Animal Molecular Breeding, 2020, Vol. 10, No. 1   
Received: 06 May, 2020    Accepted: 06 May, 2020    Published: 06 May, 2020
© 2020 BioPublisher Publishing Platform
This article was first published in Genomics and Applied Biology in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Too screen the differentially expressed ovary proteins in Dolang sheep with oestrum, anestrus and pregnancy using isobaric tag for relative and absolute quantitation (iTRAQ) technology, which will provide the basis for the research of regulation and identification of oestrum, the diagnosis of early pregnancy. In total, we identified 4847 proteins, 470 of which were significantly different proteins. Compared with anestrus, 18 proteins were up-regulated and 102 proteins were down-regulated in oestrum, 20 proteins were up-regulated and 60 proteins were down-regulated in pregnancy; Compared with oestrum, 50 proteins were up-regulated and 24 proteins were down-regulated during pregnancy. Such as cyclin-YisoformX1、tubulin alpha chain-like 3、cleavages timulation factor subunit1 isoform X1、nicotinate phosphoribosyltransferase isoform X1、integrinalpha-Mprecursor、cyclin-Y-like protein1iso form X1、cyclin-dependent kinase16 isoform X1 at up-regulated,follistatin-related protein1 isoform X2、histone H2B type2-F、heatshock protein beta-6 isoform X1 at down-regulated, which were related to hormones generation, regulation of oestrum, zygote production and apoptosis, early embryo attachment and embryo development. Of which, cyclin-Y isoform X1 and nicotinate phosphoribosyltransferase isoform X1 significant increase in pregnancy, compared with oestrum and anestrus. The study will help to identify different proteins at oestrum and pregnancy of Dolang sheep based on iTRAQ proteomics technology, which provide new thoughts and methods to further exploring the candidate biomarkers of sheep estrous identification and diagnosis of early gestation.

Dolang sheep; Ovary; iTRAQ; Proteins; Biomarkers

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Animal Molecular Breeding
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