High-throughput Identification and Marker Development of Perfect SSR for Cultivated Genus of Passion Fruit (Passiflora edulis)
1 Biotechnology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, China
2 Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, China
Molecular Plant Breeding, 2018, Vol. 9, No. 13 doi: 10.5376/mpb.2018.09.0013
Received: 05 Nov., 2018 Accepted: 07 Dec., 2018 Published: 29 Dec., 2018
© 2018 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding (2018, 20: 6738-6743) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Wu Y.Y., Tian Q.L., Liu J.Y., Huang Y.C., Huang W.H., Xia X.Z., Yang X.H., and Mou H.F., 2018, High-throughput identification and marker development of perfect SSR for cultivated genus of passion fruit (Passiflora edulis), 9(13): 92-96 (doi: 10.5376/mpb.2018.09.0013)
Simple sequence repeat (SSR) markers are characterized by high polymorphism, good reproducibility and co-dominance etc. They can be easily applied to develop efficient, simple and practical molecular markers. In the present study, bioinformatics methods were applied to identify high-throughput perfect SSRs of cultivar Passiflora genome. A total of 13,104 perfect SSRs were obtained. SSR core sequence structure is mainly 2-4 bases, the maximum numbers are TA, AT, TC and AG. The maximum numbers of repetitions were up to 20 times. A total of 12,934 pairs of SSR markers were developed by using bioinformatics software, and 20 pairs of markers were selected for amplification specificity assessment of MTX and WJ10, and the polymorphism rate was as high as 60%. The large-scale development of the SSR markers of Passiflora cultivar has paved a foundation for the efficient utilization of the germplasm resources of passion fruit, genetic improvement of the varieties and molecular breeding.
Passion fruit (Passiflora edulis); Genome; Perfect SSR; High-throughput; Molecular marker