The Screening of Ovarian Candidate Proteomics Biomarkers of Dolang Sheep at Different Physiological Periods Based on iTRAQ Technology
1 College of Animal Science, Tarim University, Alar, Xinjiang 843300, China
2 Key Laboratory of the Corps of Tarim Animal Husbandry Science and Technology, Alar, Xinjiang, 843300, China
Animal Molecular Breeding, 2021, Vol. 11, No. 1 doi: 10.5376/amb.2021.11.0001
Received: 24 Sep., 2020 Accepted: 25 Nov., 2020 Published: 08 Feb., 2021
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This article was first published in Genomics and Applied Biology in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Cui Z.L., Chang Q.Q., Yang X.L., Tuersuntuoheti M., and Chang W.H., 2021, The screening of ovarian candidate proteomics biomarkers of Dolang sheep at different physiological periods based on iTRAQ technology, Animal Molecular Breeding, 11(1): 1-8 (doi: 10.5376/amb.2021.11.0001)
To screen the differentially expressed ovary proteins in Dolang sheep with oestrum, anestrus and pregnancy using isobaric tag for relative and absolute quantitation (iTRAQ) technology, which will provide the basis for the research of regulation and identification of oestrum, the diagnosis of early pregnancy. In total, we identified 4847 proteins, 470 of which were significantly different proteins. Compared with anestrus, 18 proteins were up and 102 proteins were down in oestrum, 20 proteins were up and 60 proteins were down in pregnancy; Compared with oestrum, 50 proteins were up and 24 proteins were down during pregnancy. Such as cyclin-Y isoformX1, cleavages timulation factor subunit 1 isoform X1, follistatin-related protein 1 isoform X2, nicotinate phosphoribosyl transferase isoform X1 at up-regulated, hemopexin isoform X1, apolipoprotein A-II, nucleophosmin at down-regulated, which were related to hormones generation, regulation of oestrum, zygote production and apoptosis, early embryo attachment and embryo development. Of which, cyclin-Y isoform X1 and nicotinate phosphoribosyl transferase isoform X1 significant increase in pregnancy, compared with oestrum and anestrus. The study identified different proteins at oestrum and pregnancy of Dolang sheep based on iTRAQ proteomics technology, which provide new thoughts and methods to further exploring the candidate biomarkers of sheep estrous identification and diagnosis of early gestation.
Dolang sheep; Ovary; iTRAQ; Proteins; Biomarkers