Xiaofang Lin

Tropical Animal Medicine Research Center, Hainan Institute of Tropical Agricultural Resources, Sanya, 572000, Hainan, China
Author    Correspondence author
International Journal of Molecular Veterinary Research, 2024, Vol. 14, No. 3   
Received: 18 Jan., 2024    Accepted: 28 Feb., 2024    Published: 22 Mar., 2024

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

This study evaluates the efficacy of polymerase chain reaction (PCR) techniques in detecting various canine viral infections, including canine parvovirus (CPV), canine distemper virus (CDV), canine coronavirus (CCV), and canine parainfluenza virus (CPIV5). The study demonstrated that PCR-based methods, including nested PCR, real-time quantitative RT-PCR, and multiplex PCR, offer high sensitivity and specificity in detecting canine viral pathogens. For instance, the nested PCR technique was found to be 100 times more sensitive than single PCR in detecting CPV DNA. A duplex real-time quantitative RT-PCR assay for CPIV5 showed superior diagnostic performance compared to previous assays. Additionally, a multiplex nanoparticle-assisted PCR assay was 100-fold more sensitive than conventional PCR in detecting CDV, CPV, and CCV in mixed infections. These methods were validated using clinical samples, showing higher detection rates compared to traditional diagnostic methods. The findings suggest that PCR-based diagnostic assays are highly effective for the rapid, sensitive, and specific detection of canine viral infections. These techniques can significantly improve the accuracy of clinical diagnoses and aid in the timely management of viral diseases in dogs.

PCR; Canine viral infections; Sensitivity, specificity; Diagnostic assay; CPV; CDV; CCV; CPIV5